Genetic materials
The genetic materials used in this study included Zhengshan 97 and Minghui 63, the
parents of Shanyou 63, which is one of the best hybrids with regard to rice production in China,
and 525 F12 recombinant inbred lines (RILs) were derived from 1030 F2
plants by the single-seed
descendent method. Forty-seven inbred lines selected from the 525 RILs through the design-
breeding approach have been employed as an elite line used for molecular breeding.