Extracting solution 52, Trichloroacectic acid (TCA), 5g of TCA in 100ml of distilled water. Dye Solution: Fifty (50) mg of 2, 6-dichlorophenol-indophenol was weighed and 42mg of sodium bicarbonate was equally weighed and were dissolved in 100ml of hot (85-950C) distilled water; cooled and made to 200ml volume in volumetric flask. Standard Ascorbic acid = 50mg of ascorbic acid was weighed and made up to 10ml with 5% TCA solution. 10ml was diluted to 100ml with 5 TCA (1ML = 0.1mg of ascorbic acid).