2.3. Analyses of nutritional composition and anti-nutrient contents
The four cookies samples (15% and 30% PBF; 15% and 30% WBF replacing corn starch) were
evaluated on nutritional composition according to AOAC [14] and dietary fibre through enzymatic
method described by Freitas et al. [15] at Embrapa Rice and Beans. The thiamine (vitamin B1) and
riboflavin (vitamin B2) were evaluated by HPLC according to the European Standard methods [16, 17].
Tannins concentration was determined based on Price et al. [18] and Deshpande and Cheryan [19]
method description, using a methanol extraction and vanillin reaction with a catechin standard curve.
Phytate content was determined according to Haug and Lantzsch [20] using a spectrophotometer (Femto)
calibrated to visible absorbance range. The rice-black bean pre-gelatinized flours were also characterized
for some of those parameters.