In vitro propagation of Asparagus racemosus was carried out using shoot apex and nodal
explants in three stages. (a) Initiation of multiple shoots on MS -medium supplemented with 6-
Benzyl Adenine (8.9 μM) + Naphthalene Acetic Acid (0.27 μM) at 250C having 16 h photoperiod
(with 60 μ mol m-2 s-1 light intensity) and 8 h dark period. (b) Elongation of shoots on MS
medium supplemented with 15% coconut milk + 2-iso Pentenyl Adenine (19.6 μM), the light
intensity was increased to 80 μ mol m-2 s-1. (c) Rooting of elongated shoots by giving it a preculture
treatment with MS medium augmented with Indole Butyric Acid (7.35 μM) for 48 h and
then transfer to MS medium with 15% coconut milk. The rooted healthy plantlets were best
hardened in a mixture of 1:1 sand and soil in a moist saturated chamber having 60 -80 %
humidity. Plants were transferred to fields, after 5 wks of hardening.