were set up with each inocula.
Reaction vessel of control sets contained only media and
inocula and reaction vessel of experimental sets contained
coal along with media and inocula. Reaction vessels of all
the sets were filled up to the brim. All collection vessels
contained tap water and 200 ml of nitrogen. In each set-up
stop cocks of reaction and collection vessels were connected
together with the help of a piece of black pressure rubber
tube. Both control and experimental sets of each inocula
were incubated at 358C. The gas of the collection vessels
is analysed periodically for methane and carbon dioxide