Generally, culture and antimicrobial susceptibility testing of
N. gonorrhoeae isolates is time consuming and labor intensive.
Therefore, polymerase chain reaction (PCR)-based tests, which are
highly sensitive and specific, have been developed. However, the
antimicrobial susceptibility is not generally included in these tests.
Thus, we developed a PCR method for gonococcal identification
and also differentiation of their antimicrobial resistance. A singletube multiplex PCR specific for gonococci including a series of
primers to detect penicillin, tetracycline, andfluoroquinoloneresistant genes was developed(12). Furthermore, our method also
differentiates the specific types of plasmid-mediated resistance to
penicillin (Asia, Africa, and Toronto), and tetracycline (American
and Dutch). This method is suitable to be used in the routine laboratory to reduce the time-consuming and labor-intensive standard
methods for the detection of gonococci and its antibiotic
susceptibility.
Generally, culture and antimicrobial susceptibility testing ofN. gonorrhoeae isolates is time consuming and labor intensive.Therefore, polymerase chain reaction (PCR)-based tests, which arehighly sensitive and specific, have been developed. However, theantimicrobial susceptibility is not generally included in these tests.Thus, we developed a PCR method for gonococcal identificationand also differentiation of their antimicrobial resistance. A singletube multiplex PCR specific for gonococci including a series ofprimers to detect penicillin, tetracycline, andfluoroquinoloneresistant genes was developed(12). Furthermore, our method alsodifferentiates the specific types of plasmid-mediated resistance topenicillin (Asia, Africa, and Toronto), and tetracycline (Americanand Dutch). This method is suitable to be used in the routine laboratory to reduce the time-consuming and labor-intensive standardmethods for the detection of gonococci and its antibioticsusceptibility.
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