Preliminary studies (data not shown) of 72-h batch fermentation have shown that an optimal ethanol concentration of 98.6 g/L with an ethanol yield (YP/S) of 0.47 (g/g) are obtained by TSC-immobilized cells (TSC-cell) at an initial reducing sugar concentration of 240 g/L. The ethanol concentration and conversion yield of the immobilized cells were 11.5% and 9.3% higher than those of the free cells. The inhibitory effect of high sugar concentration on ethanol fermentation by free cells has been previously reported [14]. It was found that, at high initial sugar concentrations, the system using TSC-cells was more efficient than when using free cells.