IntroductionVinegar has been widely used in food industry [1]. Global vine-gar demand is increasing significantly in the recent ten years(http://www.versatilevinegar.org). Producing high acidity vinegar(acidity ≥ 90 g/L acetic acid) is one of the solutions to meet marketdemand, and it can also save storage and transport cost. Acetobac-ter as the main genus is involved in conventional rice wine vinegarproduction where acidity commonly does not exceed 60 g/L [2–4].At present, few Aectobacter species were applied in high acidity ricewine vinegar production. So, how to ferment high acidity vinegarby Acetobacter species is valuable.Vinegar is formed by the stoichiometric conversion of ethanolwith oxygen to acetic acid and water by acetic acid bacteria (AAB)[5]. The advanced technology for this industry is based on the sub-merged fermentation in semi-continuous mode [6,7]. It consistsof the developing of successive repeated acetification processes:when the concentration of alcohol reaches minimum residuallevel (depending on the type of vinegar), a quantity of mediumis discharged and refilled with the same volume of fresh mediumcontaining a certain amount of alcohol; the residual culture broth