The effects of chitosan on mycelial growth of B. cinerea and
P. expansum were assayed according toYao andTian (2005). The
mycelial disks (5mm in diameter) from 2-week-old cultures of
the fungi were placed in the centre of Petri dishes (90mm in
diameter) with 20mL of PDA containing different concentrations
of chitosan (0, 0.01, 0.05, 0.1, 0.5 and 1%), then incubated
at 25 ◦C. The mycelial growth was determined by measuring