ACC deaminase activity was assayed according to a modified
method of [22,23] which measures the amount of α-ketobutyrate
produced upon the hydrolysis of ACC. The number of μmol of
α-ketobutyrate produced by this reaction was determined by
comparing the absorbance at 540 nm of a sample to a standard
curve of α-ketobutyrate ranging between 10 and 200 μmoL.