sequencingBands of purified STAP enzyme were separated on SDS gelsand transferred to a ProBlott membrane (Applied Biosystems, Fos-ter City, CA, USA), and the NH2-terminal sequence analysis wasperformed by automated Edman’s degradation using an AppliedBiosystem Model 473A gas-phase sequencer per standard operat-ing procedures. Amino acid residues were detected as individualsignals.