Diagnosis of serotype was attempted on acute serum samples of 394 children who had serological confirmed dengue virus infection. These tests yielded DENV from 333 (84.5%) children, and the detection rate varied with only 67% in 2006 using mosquito isolation compared to 83% in 2007, 86% in 2008, and 96% in 2009 using RT-PCR. Viral detection also varied by day of sample collection, the highest rate (95%) was from the samples collected on days 1 and 2 after onset of fever, followed by days 3 and 4 (89%) and days 5 and 6 (69%).