The mixed culture of P. fermentans and C. stella with S. cerevisiae could generate higher levels of esters, fatty acids and norisoprenoids compared to the monoculture of S. cerevisiae, and a co-culture of M. pulcherrima, I. orientalis and H. uvarum could accumulate higher amounts of higher alcohols. Our results suggested that non- Saccharomyces yeast strains greatly contributed to the fruity aroma quality of SF wine, and the mixed culture of Saccharomyces yeasts with non-Saccharomyces yeasts such as P. fermentans, C. stella or M. pulcherrima is a valuable tool to modulate the volatile profiles and improve the aromatic complexity of wine.