DPPH radical is a stable organic free radical with an absorption
band at 517 nm. It loses this absorption when accepting an
electron or a free radical species, which results in a visually
noticeable discoloration from purple to yellow. It can accommodate
many samples in a short period and is sensitive
enough to detect active ingredients at low concentrations [25].
Fig. 3 shows the DPPH scavenging activities of the extracts in a
concentration-dependent manner. The extract obtained by
100% ethanol yielded the highest DPPH radical scavenging
activity at concentrations ranging from 60 mg/mL to 180 mg/mL.