A new fungus was isolated locally from rotten maize and
identified as Rhizopus oryzae RCK2012 . It was cultivated and maintained on potato dextrose
agar (PDA). Inoculum was prepared from 3 days old slant by
suspending the fungal spores in sterile distilledwater and adjusted
to a concentration of 1106 spores/ml. One batch of commercial
wheat grains were stored at room temperature and were used
throughout the experiments.