The treatments with 128.3 mM and higher
concentrations exhibited browning and did not show
any sign of regeneration in the first subculture cycle.
However, in a subsequent subculture, regeneration was
obtained in 10 and 20 Gy irradiated calli on 128.3 and
171.1 mM NaCl selection medium. In 40 Gy irradiated
calli, regeneration could be seen on 85.6 mM NaCl
selection. However no regeneration was observed on
salt selection in case of 30 and 50 Gy irradiated calli
(Fig.4). A total of 147 plantlets were selected from
different salt selection media. The unirradiated calli
regenerated the highest number of plantlets followed
by 10 and 20 Gy irradiated calli on salt selection.