Briefly, total RNAs were extracted by using the RNeasy kit (Qiagen,Valencia, CA) following manufacturer’s instructions. The cDNAswere synthesized with the Uni12 primer and avian myeloblastosisvirus (AMV) reverse transcriptase (Promega, Madison, WI) accord-ing to manufacturer’s instructions. And the cDNAs were stored at−80◦C until use.