In conclusion, the present study demonstrated that the n-3 PUFA concentrations in crayfish diet had a positive effect on the quality of fatty acids, cholesterol and fat-soluble vitamins of the abdomen meat in both female and male A. leptodactylus. The best fatty acid composition, cholesterol and fat-soluble vitamin contents in the abdomen meat of A. leptodactylus were obtained from the crayfish fed D4. However, the fat-soluble vitamin levels in the abdomen meat of both sexes A. leptodactylus (except retinol, retinol acetate and δ-tocopherol levels) were unaffected by the dietary n-3 PUFA.