Sample thermaldecompositionfollowedbymercuryamalgamationandatomicabsorptionhasbeen
employedforthedeterminationofmethylmercury(MeHg)in fish. ThemethodinvolvesHBrleachingof
MeHg, extractionintotoluene,andback-extractionintoanaqueous L-cysteinesolution.Preliminary
studies werefocusedontheextractionefficiency,losses,contaminations,andspeciesinterconversion
prevention.Thelimitofdetectionwas0.018 mg g1 (dry weight).Theintradayprecisionforthree
replicateanalysisataconcentrationof4.2 mg g1 (dry weight)was3.5percent,similartotheinterday
precision accordingtoanalysisofvariance(ANOVA).Theaccuracywasguaranteedbytheuseoffortified
samples involving83–105percentrecoveries,andcertified referencematerialsTORT-2(lobster
hepatopancreas)andDORM-3(dogfish liver),providing107and98percentrecoveryofcertified values.
The greennessofthemethodwasalsoevaluatedwiththeanalyticaleco-scalebeingobtaineda final
score of73pointswhichmeansanacceptablegreenanalysis.Themethodwasappliedto fifty-seven
market samplesofdifferent fish acquiredfromlocalmarketsinseveralsamplingcampaigns.Thecontent
of MeHgfoundvariedbetween0.0311and1.24 mg g1 (wet weight),withvaluesthatinvolve33–129
percent ofthetotalmercurycontent.Someconsiderationsaboutfoodsafetywerealsodonetakinginto
account dataaboutSpanish fish consumeandTolerableWeeklyIntake(TWI)establishedforMeHg.
Sample thermaldecompositionfollowedbymercuryamalgamationandatomicabsorptionhasbeenemployedforthedeterminationofmethylmercury(MeHg)in fish. ThemethodinvolvesHBrleachingofMeHg, extractionintotoluene,andback-extractionintoanaqueous L-cysteinesolution.Preliminarystudies werefocusedontheextractionefficiency,losses,contaminations,andspeciesinterconversionprevention.Thelimitofdetectionwas0.018 mg g1 (dry weight).Theintradayprecisionforthreereplicateanalysisataconcentrationof4.2 mg g1 (dry weight)was3.5percent,similartotheinterdayprecision accordingtoanalysisofvariance(ANOVA).Theaccuracywasguaranteedbytheuseoffortifiedsamples involving83–105percentrecoveries,andcertified referencematerialsTORT-2(lobsterhepatopancreas)andDORM-3(dogfish liver),providing107and98percentrecoveryofcertified values.The greennessofthemethodwasalsoevaluatedwiththeanalyticaleco-scalebeingobtaineda finalscore of73pointswhichmeansanacceptablegreenanalysis.Themethodwasappliedto fifty-sevenmarket samplesofdifferent fish acquiredfromlocalmarketsinseveralsamplingcampaigns.Thecontentof MeHgfoundvariedbetween0.0311and1.24 mg g1 (wet weight),withvaluesthatinvolve33–129percent ofthetotalmercurycontent.Someconsiderationsaboutfoodsafetywerealsodonetakingintoaccount dataaboutSpanish fish consumeandTolerableWeeklyIntake(TWI)establishedforMeHg.
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