Filtered extracted solutions were concentrated using a rotary
evaporator (40 ◦C). Each dried extract was dissolved in initial
HPLC mobile phase (CH3CN/H2O (20/80, v/v)) at a concentrations
(m/v) of 5mg/mL and filtered through 0.45mm Nylon micropore
membranes. Ten microliter were injected for HPLC–DAD–ESI/MSn
analysis and for each sample three independent extractions were
performed.