In total, there were nine treatment combinations: (1)
control without P (?P) and without actinomycetes; (2)
(?P) + isolate # 18; (3) (?P) + isolate # 5; (4) with SP addition
(+SP); (5) with PRP addition (+PRP); (6) (+SP) + isolate # 18; (7)
(+SP) + isolate # 5; (8) (+PRP) + isolate # 18; and (9) (+PRP) + i-
solate # 5. Treatments 1–3 did not receive any P source. Bean
seeds were surface-sterilized in 70% ethyl alcohol for 3 min
followedbyimmersionin1.05%NaOCl(20%householdbleach)
for 4 min. Surface-sterilized seeds were then washed 10 times
with sterile distilled water. Three weeks after preparing the
soils with the required treatments, seven seeds were sown in
each pot to a depth of 5 mm. When emergence was complete
(ca.5days)theseedlingswerethinnedto4perpotandthepots
were placed in an evaporative-cooled greenhouse at 27 ? 3 8C.
The pots were watered daily to container capacity and after 10
days from sowing, micronutrients were added as chelates
with EDTA at the rate of 3.5, 1.45, and 1.45 kg ha ?1 ,
respectively, of Fe, Mn and Zn. Container capacity was
determined by filling seven replicate free-draining pots with
8 kg of soil. The soil was slowly saturated with water and then
allowed to drain overnight until no more drained from the
pots. The soil was then re-weighed to determine container
capacity.The mean weigh to fwater lost was then applied to all
pots on a daily basis.
In total, there were nine treatment combinations: (1)control without P (?P) and without actinomycetes; (2)(?P) + isolate # 18; (3) (?P) + isolate # 5; (4) with SP addition(+SP); (5) with PRP addition (+PRP); (6) (+SP) + isolate # 18; (7)(+SP) + isolate # 5; (8) (+PRP) + isolate # 18; and (9) (+PRP) + i-solate # 5. Treatments 1–3 did not receive any P source. Beanseeds were surface-sterilized in 70% ethyl alcohol for 3 minfollowedbyimmersionin1.05%NaOCl(20%householdbleach)for 4 min. Surface-sterilized seeds were then washed 10 timeswith sterile distilled water. Three weeks after preparing thesoils with the required treatments, seven seeds were sown ineach pot to a depth of 5 mm. When emergence was complete(ca.5days)theseedlingswerethinnedto4perpotandthepotswere placed in an evaporative-cooled greenhouse at 27 ? 3 8C.The pots were watered daily to container capacity and after 10days from sowing, micronutrients were added as chelateswith EDTA at the rate of 3.5, 1.45, and 1.45 kg ha ?1 ,respectively, of Fe, Mn and Zn. Container capacity wasdetermined by filling seven replicate free-draining pots with8 kg of soil. The soil was slowly saturated with water and thenallowed to drain overnight until no more drained from thepots. The soil was then re-weighed to determine containercapacity.The mean weigh to fwater lost was then applied to allpots on a daily basis.
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