Sweet corn is an important economic crop of the world for sale in fresh ear and processing in can. At the present nutrition value from food is more consider by consumer. Purple opaque-2 sweet corn is an alternative for consumer because of more anthocyanin in kernel and more tryptophan in endosperm. In addition, anthocyanin is antioxidant that can reduce free radical, anti-inflammatory and reduce cholesterol. The objective in this study will be increase tryptophan content in kernel of purple sweet corn using marker assisted-selection of opaque-2 gene in the segregating population. S4 line of purple opaque-2 sweet corn (Pr_C_sh2sh2o2o2) will be used as donor parent crossing with Insee 2 (Pr_ccsh2sh2O2O2), yellow sweet corn controlled by shrunken 2 gene obtaining F1 hybrid. The S1 seed will be obtained by self-pollination of F1 plant. Then, marker assisted-selection, phi057, will be applied to detect the opaque-2 plant (o2o2) from S1 segregating population. The selected opaque-2 plants will be grown in the field for advanced generation until S3. The cross will be made between 20 lines of yellow opaque-2 sweet corn and 2 testers of purple opaque-2 sweet corn by line × tester method getting 40 crosses. Yield trial will be conducted in randomized complete block design with 3 replications. The analysis of tryptophan content in endosperm and anthocyanin content in fresh kernel will be done at the age of kernel about 21 days after pollination in both parental lines and their F1 hybrids. Moreover, general combining ability (GCA) and specific combing ability (SCA) for ear fresh weight and some agronomic characters will be analyzed using R statics program. The expected result will be the hybrid of purple opaque-2 sweet corn containing high antioxidant from anthocyanin content and high tryptophan content in fresh kernel.