Test sample preparation for Brine shrimp bioassay
Test sample was dissolved in DMSO (Dimethyl sulfoxide) to obtain stock solution of 1 mg/mL concentration. The final concentration of DMSO in the assay volume was kept at 2% to prevent possible false effects originating from DMSO toxicity. Pure DMSO and artificial seawater were used as negative control and potassium dichromate was used as the reference standard for the cytotoxicity assay.