Research on new sources of bioenergy is nowadays driving attention to microalgae. Cost-effective biomass harvesting
and thickening pose a challenge for massive microalgae production for biofuels. In this study, . Finally, the potential toxicity of flocculants upon biomass production was
assessed in biochemicalmethane potential tests. Results indicated that doses of 10–50 mg/L of Ecotan and Tanfloc
did not affect anaerobic digestion, leading to the same methane yield (162–166 mL CH4/g VS) with the same
methane content (70%) as the control without flocculants. This study demonstrates that Ecotan and Tanfloc flocculants
would be appropriate for microalgae biomass harvesting and subsequent biogas generation.