2.1. Sample origin and pre-treatment
The experiment was done on laboratory scale, with freshly caught wild young YFT purchased from long line fishermen in Latuhalat, Ambon. The total length of samples bought was measured in situ directly after purchase. Samples were eviscerated and remaining body liquids were drained as thoroughly as possible to reduce bias due to microbial contaminants from the internal organs. Individual weight of each sample was measured before and after the evisceration process. Samples were placed in individual clean transparent plastics and arranged in a clean storage box so as to minimize the piling effect and treated immediately with nano-chitosan upon arrival to the laboratory. No ice was added to reduce microbial contamination source, but the storage box environment was conditioned to be as cool as possible (between 10 °C and 18 °C) during transport.