The inhibition zone diameters for active bioplastics containing two different oregano essential oil concentrations (5 and 10 wt.%) are presented in Table 2. As previously explained, K-B tests conducted
on the WG-bioplastic without addition of the biocide revealed the absence of antimicrobial activity. However, bioplastics containing this bioactive agent have demonstrated a suitable antimicrobial activity, which becomes higher as its concentration increases. Furthermore, at 5 wt.% OEO, the biocide effectiveness is clearly higher when this is incorporated into a WG-based bioplastic. Previous results revealed that a suitable combination of formulation and processing conditions may lead to materials with different microstructure, which was seen to ultimately control their linear viscoelastic response. In the same manner, microstructure might also influence the way a specific active agent diffuses out of the bioplastic matrix. Hence, inhibition diameters shown in Table 2 verify that WG-based matrix would be based on a microstructure which seems to facilitate the biocide migration. Finally, it can be observed that, on the contrary to formic acid, oregano essential oil seems to exert a greater antimicrobial effect on A. niger after being incorporated into the bioplastic. E. coli and C. kefyr are the most resistant microorganism under the action of oregano essential oil at 5 wt.% OEO and for the EW matrix. Interestingly, essential oil may also inhibit the growth of microorganisms even if it is not in direct contact with them, by promoting
the formation of an antimicrobial atmosphere within the container.
The inhibition zone diameters for active bioplastics containing two different oregano essential oil concentrations (5 and 10 wt.%) are presented in Table 2. As previously explained, K-B tests conductedon the WG-bioplastic without addition of the biocide revealed the absence of antimicrobial activity. However, bioplastics containing this bioactive agent have demonstrated a suitable antimicrobial activity, which becomes higher as its concentration increases. Furthermore, at 5 wt.% OEO, the biocide effectiveness is clearly higher when this is incorporated into a WG-based bioplastic. Previous results revealed that a suitable combination of formulation and processing conditions may lead to materials with different microstructure, which was seen to ultimately control their linear viscoelastic response. In the same manner, microstructure might also influence the way a specific active agent diffuses out of the bioplastic matrix. Hence, inhibition diameters shown in Table 2 verify that WG-based matrix would be based on a microstructure which seems to facilitate the biocide migration. Finally, it can be observed that, on the contrary to formic acid, oregano essential oil seems to exert a greater antimicrobial effect on A. niger after being incorporated into the bioplastic. E. coli and C. kefyr are the most resistant microorganism under the action of oregano essential oil at 5 wt.% OEO and for the EW matrix. Interestingly, essential oil may also inhibit the growth of microorganisms even if it is not in direct contact with them, by promotingthe formation of an antimicrobial atmosphere within the container.
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