The column was eluted at 35 C with a
binary gradient of 100% solution A [9% acetonitrile, 2% acetic acid,
containing EDTA (20 mg/ml)] for 10 min, 68% of solution A and 32%
of solution B [80% acetonitrile, 2% acetic acid, containing EDTA
(20 mg/ml)] for 10 min at a flow rate of 1.0 ml/min. The eluent
was monitored at 278 nm.