The cellular deaths observed in 2nd, 4th and 6th runs may be
attributed to the high WE concentration. Lim et al. (2010)
studied the cultivation of C. vulgaris, from textile waste effluent;
they stated that the dilution of the textile waste effluents
is an important factor affecting the algal growth and biomass
productivity. Moreover C. vulgaris grew in 100% waste effluent;
although the final biomass attained was significantly lower
(p< 0.05) than that grown in 20–80% textile waste concentration.
However, Phang and Chu (2004) reported that C. vulgaris
UMACC 001 was shown to be a versatile alga that is able to
grow under various harsh conditions.
In this study C. vulgaris succeeded in decolorizing the WE
during all the studied runs. In this respect, Acuner and Dilek
(2004) reported that several species of Chlorella were capable
of degrading azo dyes to their aromatic amines and to further
metabolize the aromatic amines to simpler organic compounds
or CO2 and thereby detoxifying them. Furthermore, El-Sheekh
et al. (2009) reported the ability of C. vulgaris to decolorize a
variety of azo dyes via algal azo dye reductase enzyme.