The present study showed the capacity of probiotic bacteria to reduce the bioaccessibility of AFB1 and AFB2 in spiked loaf bread. In particular, the highest bioaccesibility AFs reduction was obtained when the spiked loaf bread was digested together with Lb. johnsoni CECT 289, Lb. casei CECT 4180, Lb. plantarum CECT 220 and Lb.reuteri CECT 725, reaching reduction up to 98.66%.Results from this study suggest that a food enriched with specific probiotic microorganisms and consumed at the same time as food contaminated with AFs, could reduce the risk associated to the intake of these toxic compounds contained in food.