Malaria remains to be one of the serious problems in tropical countries because of the increase in
antimalarial drug resistance. This investigation was to study the extraction of bioactive compounds with
anti-malarial activity from Yanang (Tiliacora triandra Diels) root. The dried root was extracted with
chloroform : methanol : ammonium hydroxide mixture (50:50:1). Further isolation and purification of the
crude extract using column chromatography and crystallization techniques provided two pure alkaloid
compounds: tiliacorinine (I) and tiliacorine (II) with 0.0082 and 0.0029 percent yield, respectively.
Structures of I and II were confirmed by spectroscopy techniques and compared with reference data.