The specificity (cross-amplification) of real-time RT-PCR in Fig. 3d
shows that no amplification was observed using total genomic RNA
fromthe shrimp infectedwith TSV, YHV, IMNV, and no template control
(NTC) (Fig. 3d), which was confirmed by electrophoresis (Fig. 3e). This
also indicates that the amplifications of the primer sets are specific to
the target virus and clearly amplified.