Turnover of the K’ transport system in Saccharomyces cerevisiae
The stability of the K’ transport system in Sacchurornyces cerevisiae has been studied upon inhibition of protein synthesis with cycloheximide.
Addition of the antibiotic gave rise lo an inactivation of this transport. This activation followed first-order kinetics and was stimulated by the
presence of a fermentable substrate. A half-life of about 4 h could be calculated in the presence of glucose. The results indicate that, similarly to
sugar carriers, K’ transport system is less stable than the bulk of proteins of this organism.
K’ transport; Protein turnover; Plasma membrane ATPase; Saccharomyces cerevisiae