We applied this extraction procedure to a range of bacterial species of interest in veterinary medicine to examine its usefulness for the extraction and purification of components of value in diagnostic or vaccine applications. The bacteria used were a rough gram-negative species (Brucella ovis) , three smooth gram-negative
species (Brucella abortus, Yersinia enterocolitica serotype 0:9 and Bordetella bronchiseptica), a gram-positive species (Corynebacterium pseudotuberculosis)
and a mycobacterium (M.paratuberculosis). B. ovis is the major causative agent of ovine contagious epididymitis, a disease which results in reduced fertility in rams .
Infections with B. abortus are an important cause of abortions in cattle worldwide.