Amino acid composition was determined based on Zheng Lin’s
method with modifications (Zheng et al., 2012), using an A300 auto
amino acid analyzer (membra Pure, Bodenheim, Germany). Briefly,
sample solutions were filtrated through a 0.22 lm membrane filter
and then directly injected into the analyzer for the free amino acid
determination. Whereas, the total amino acid composition of the
sample was determined after the hydrolysis at 110 C for 24 h with
6 M hydrochloric acid. In parallel, alkaline hydrolysis at 105 C for
24 h with 4 M NaOH was also performed to determine the tryptophan
concentration. The amount of each amino acid was expressed
as mg/g (the dry weight of non-salt soluble solids (NSSS) in soy
sauce).
Amino acid composition was determined based on Zheng Lin’smethod with modifications (Zheng et al., 2012), using an A300 autoamino acid analyzer (membra Pure, Bodenheim, Germany). Briefly,sample solutions were filtrated through a 0.22 lm membrane filterand then directly injected into the analyzer for the free amino aciddetermination. Whereas, the total amino acid composition of thesample was determined after the hydrolysis at 110 C for 24 h with6 M hydrochloric acid. In parallel, alkaline hydrolysis at 105 C for24 h with 4 M NaOH was also performed to determine the tryptophanconcentration. The amount of each amino acid was expressedas mg/g (the dry weight of non-salt soluble solids (NSSS) in soysauce).
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