2. Materials and methods
2.1. Reagents
Phosphate buffer saline (PBS), penicillin-streptomycin and DMEM
were from Invitrogen.
25 cm3 Flasks and 96-well microplates were from Nunc, fetal bovine serum (FBS) was from Seromed, Tris was from Gibco BRL, Sulforhodamine B (SRB) was from Sigma, trichloroacetic acid (TCA) was from Carlo Erba. The cell lines were from the Cell Lines