Selection of wavelength
Scan standard solution in UV spectrophotometer between 200 nm
to 400 nm on spectrum mode, using diluents as a blank (Figure 1).
Paracetamol shows λmax at 243. The proposed analytical method is
simple, accurate and reproducible (Figure 2).
Method validation
Specificity: Resolution of the analyte peak from the nearest peak:
Solution of each of the analyte was injected separately and their retention
time is noted. The standard working solution containing a mixture of
the component being analyze is also injected and each of analyte peaks
is check for its resolution from the nearest (Figures 2,3,4,5).
Linearity: Six points calibration curve were obtained in a
concentration range from 0-150 ppm for Paracetamol. The response
of the drug was found to be linear in the investigation concentration
range and the linear regression equation was y = 0.004x+0.007 with
correlation coefficient 0.998 (Table 2, Figure 6).