We have adapted a thin-layer chromatography (TLC)
double-beam densitometer to accept 96-well microtiter
plates for determination of reducing sugars by a modified
Nelson-Somogyi method. Each well is read by reflec-
tance, thus avoiding the time-consuming filtration step
necessary in the standard Nelson-Somogyi assay in
cases when insoluble precipitate hinders reading by ab-
sorbance (transmission)