Protection against CMV was evaluated biologically by
studying a number of asymptomatic plants and developed
symptom degree. Virus absence was proved serologically by
I-ELISA and molecularly by IC-RT-PCR. Azotobacter followed
by Pseudomonas irrigation crude culture treatment gave
remarkable results by decreasing symptom degree and production
of an acceptable number of virus free-plants. However,
using of IC-RT-PCR revealed that just one of the I-ELISA
negative plants gave positive PCR results in the form of a band
of CMV cp gene proving that the bacterial treatment may inhibit
the virus infection and replication with a great extent.