abstract
Green banana flour (GBF) was obtained from green banana fruit submitted to a previous limewater deastringency
treatment. Autoclaved/debranched GBF (ADGBF) was prepared by autoclaving and
debranching GBF with different levels of pullulanase to induce formation of resistant starch (RS) type III.
The soluble condensed tannin (SCT), chemical composition, in vitro starch digestibility and estimated
glycemic index (eGI) were evaluated. SCT was reduced significantly by limewater de-astringency treatments
(p < 0.05). An optimal level of pullulanase for debranching was determined. GBF and ADGBF
contained similar amounts of crude protein, crude fat and total starch, however they differed in RS and
total dietary fiber (TDF) contents; RS was higher in GBF than in ADGBF, but TDF was lower in GBF, since
RS that survives boiling during analysis can be assessed by determining TDF, resulting in increased
thermal stability of enzyme resistance in ADGBF. Kinetics of in vitro starch hydrolysis indicated the rate
and extent of starch hydrolysis and the glycemic response were different between ADGBF and its
parental flour. ADGBF had higher hydrolysis index (HI) and eGI than its parental flour, suggesting that RS
had an obvious impact on duration of the glycemic response, and thus on the HI and eGI values.
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