The determination of the caffeine content of the coffee fruit
peels was carried out according to a modification of the Adolfo
Lutz Institute method used by Yoshida et al. (2005). For the calculation
of the caffeine content (Cce), the caffeine concentration in
the solution (Ccs) was determined by spectrophotometer readings.
The following equation was used: Cce(%) = (Ccs·Vs/Mp)100 in which
Vs is the solution volume and Mp represents the total coffee fruit
peel mass.
then the total volume was brought to 2.5 mL with distilled water.
The samples were shaken in a vortex shaker and the absorbance
measured at 510 nm. All the tests were done in triplicate and the
results expressed in mg rutin/g extract.
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