According to the method of Thomas, Crepeau, Rumpunen, and Thibault (2000) with slight modifications, defatted seed sample was homogenized in 85% (v/v) boiling alcohol (seed:alcohol 1:30 w/v), using the Osterizer at the ‘‘Hi’’ speed for 1
min. The suspension was further boiled for another 40 min and the sample was extracted twice. AIS was filtered, washed with 70% ethanol, and dried by solvent exchange and air at 30 C.