The supernatant containing the enzyme (0.60 mL) was collected and mixed with 1.00 mL of phosphate-citrate buffer pH 5.0 containing guaiacol (0.20 mol/L) and 0.10 mL of hydrogen peroxide (0.3% v/v).
The supernatantcontaining the enzyme (0.60 mL) was collected and mixedwith 1.00 mL of phosphate-citrate buffer pH 5.0 containing guaiacol(0.20 mol/L) and 0.10 mL of hydrogen peroxide (0.3% v/v).