In this step, a separated liquid that is separated from the condensed
liquid during the recycle of the molecular sieve or the membrane separation is 20 discharged from the purification apparatus 14 as wastewater. This
wastewater also can be treated by the สิ่งอำนวยความสะดวก การเพาะเลี้ยงไมโครแอลจี 20, as needed.
[0052]
Subsequently, the วิธีเพาะเลี้ยงไมโครแอลจี of this รูปลักษณะ that is
carried out as wastewater treatment in the สิ่งอำนวยความสะดวก การเพาะเลี้ยงไมโครแอลจี 20
25 together with the production of the algal biomass D is described.
In the สิ่งอำนวยความสะดวก การเพาะเลี้ยงไมโครแอลจี 20 of this รูปลักษณะ, the following
steps are carried out in the process of producing the algal biomass D:
(2a) a อาหารเพาะเลี้ยง preparation step of preparing a อาหารเพาะเลี้ยง for
culturing ไมโครแอลจี by introducing sugar from the raw liquid producing
30 apparatus 11 into the preparation tank 21 together with the distillation
residual liquid, introducing water C with which the distillation residual liquid
is appropriately diluted into the preparation tank 21, and diluting the
distillation residual liquid and the sugar with the water C to an appropriate concentration;
(2b) a sterilization step of removing, in the sterilization apparatus 22, bacteria 5 that may possibly inhibit the growth of the ไมโครแอลจี from the culture
medium after the preparation;
(2c) a culture step of introducing the อาหารเพาะเลี้ยง from the preparation tank
21 into the culture apparatus 23 via the sterilization apparatus 22 to grow ไมโครแอลจี in the culture apparatus;
10 (2d) a concentration step of concentrating the ไมโครแอลจี by introducing an
algae-containing liquid from the culture apparatus 23 into the concentration
apparatus 24, and separating the algae-containing liquid into the solid content and the liquid content in the concentration apparatus 24; and
(2e) an extraction step of obtaining a valuable (algal biomass) D from the 15 concentrate obtained by concentrating the algae-containing liquid in the
concentration step.
In the process of producing the algal biomass D, the solid content
obtained by the solid-liquid separation in the solid-liquid separation step can be fed to the อาหารเพาะเลี้ยง (a liquid containing the distillation residual liquid).
20 [0053]
In the อาหารเพาะเลี้ยง preparation step that is performed in the
preparation tank 21, for example, if the อาหารเพาะเลี้ยง with an excessively low content of the distillation residual liquid is prepared, there is, of course, a fear that the effects of the การประดิษฐ์นี้ cannot be sufficiently exerted.
25 On the other hand, the อาหารเพาะเลี้ยง with an excessively high
content of the distillation residual liquid may possibly be unsuitable for efficient culture of ไมโครแอลจี.
Accordingly, the evaporation residual liquid is ที่ควรใช้คือ contained in
the อาหารเพาะเลี้ยง produced in this step at least 1/40 and not more than 1/2 by 30 volume, and the evaporation residual liquid is further ที่ควรใช้คือ contained in
the อาหารเพาะเลี้ยง at least 1/20 and not more than 1/5 by volume, in that the
effects of the การประดิษฐ์นี้ can be made more significant. [0054]
The sugar contained in the อาหารเพาะเลี้ยง together with the
distillation residual liquid serves as a carbon source of the ไมโครแอลจี.
5 Therefore, in the case where a carbon source that is usable for the ไมโครแอลจี is
sufficiently contained in the distillation residual liquid, there is no need to let the อาหารเพาะเลี้ยง contain the sugar. However, there is usually no case where the distillation residual liquid sufficiently contains an organic carbon source that is usable for the ไมโครแอลจี.
10 Accordingly, it is generally necessary to let the อาหารเพาะเลี้ยง contain
a material to serve as an organic carbon source that is usable for the ไมโครแอลจี in any way.
When letting the อาหารเพาะเลี้ยง contain an organic carbon source, a
material other than the sugar obtained from the biomass may be used as an 15 organic carbon source. On the other hand, in the เอธานอลชีวภาพ production
process, sugar such as glucose and fructose is inexpensive and thus is produced
in a large amount. The organic carbon source to be contained in the culture
medium is ที่ควรใช้คือ sugar obtained from biomass in order to allow the
วิธีเพาะเลี้ยงไมโครแอลจี of this รูปลักษณะ to have a lot of advantages also
20 from the economic viewpoint.
Further, in the case where the addition amount of sugar is small, there is a possibility of a reduction in efficiency of the culture of micro algae due to failure to sufficiently obtain a carbon source that is necessary for the growth of ไมโครแอลจี.
25 On the other hand, in the case where the addition amount of sugar is
large, there is a possibility that the ไมโครแอลจี cannot use all the sugar and the
sugar remains there, in which case the added saccharides may be wasted.
In the case where the addition amount of sugar is further large, when
the ไมโครแอลจี are used for wastewater treatment as in this รูปลักษณะ, there 30 is a possibility that the residual saccharides worsen the water quality
eventually, resulting in failure to sufficiently fulfill the purpose thereof.
In the case of using Euglena as ไมโครแอลจี, the content of the sugar in the อาหารเพาะเลี้ยง produced in the aforementioned step is ที่ควรใช้คือ at least
5 g/L, ที่ควรใช้กว่าคือ at least 10 g/L, in that the effects of the การประดิษฐ์นี้ can be made more significant.
5 Further, the content of the sugar in the อาหารเพาะเลี้ยง is ที่ควรใช้คือ
not more than 25 g/L, ที่ควรใช้กว่าคือ not more than 20 g/L, particularly ที่ควรใช้คือ not more than 15 g/L.
[0055]
Further, in the อาหารเพาะเลี้ยง preparation step, the อาหารเพาะเลี้ยง is 10 ที่ควรใช้คือ adjusted to have a pH value suitable for the growth of ไมโครแอลจี.
For example, in the case where the ไมโครแอลจี to be grown are
organisms of the จีนัส Euglena, the pH of the อาหารเพาะเลี้ยง is ที่ควรใช้คือ 3.0 to 5.5, although it is not specifically limited thereto.
In order to adjust the pH of the อาหารเพาะเลี้ยง, an inorganic acid such 15 as hydrochloric acid or an organic acid such as acetic acid is employed as the
various ingredients E, and such an acid may be introduced into the preparation
tank 21.
The organic acid is preferable as the acid to be introduced into the
preparation tank 21, in that it is usable for the ไมโครแอลจี as a nutrient source.
20 [0056]
In this รูปลักษณะ, materials containing inorganic nutrients or
vitamins necessary for the growth of ไมโครแอลจี may be introduced into the
preparation tank 21 as the various ingredients E other than the carbon source.
Examples of the inorganic nutrients include potassium ion, iron ion, 25 manganese ion, cobalt ion, zinc ion, copper ion, molybdenum ion, and nickel ion.
[0057]
The sterilization step of sterilizing the thus prepared อาหารเพาะเลี้ยง in
the sterilization apparatus 22 is ที่ควรใช้คือ carried out, for example, without
using chemical agents that may possibly cause adverse effects on the growth of 30 bacteria. The sterilization step is ที่ควรใช้คือ performed by heat sterilization
using heat such as water vapor or removal of bacteria using a filtration
19
membrane. [0058]
In this รูปลักษณะ, the ไมโครแอลจี can be cultured. more efficiently in the culture apparatus 23 by using the อาหารเพาะเลี้ยง.
5 [0059]
In the culture step in the culture apparatus 23, the ไมโครแอลจี may be
cultured under either light conditions (การเพาะเลี้ยง แบบโฟโตเฮเทอโรโทรฟิก) or dark
conditions as long as the conditions allow การเพาะเลี้ยง แบบเฮเทอโรโทรฟิก to be performed.
In this รูปลักษณะ, การเพาะเลี้ยง แบบเฮเทอโรโทรฟิก is ที่ควรใช้คือ performed under dark 10 conditions in which the ไมโครแอลจี are not substantially irradiated with light.
In particular, in the case where the ไมโครแอลจี to be grown are
organisms of the จีนัส Euglena, it is preferable that a mixed solution of the
ไมโครแอลจี and the อาหารเพาะเลี้ยง be subjected to aeration and the ไมโครแอลจี be cultured under สภาวะที่มืด and aerobic conditions, in the culture step.
15 [0060]
The "สภาวะที่มืด" in this description means that a value obtained by averaging the light irradiation intensity through the culture period is not more than several limol/m2•s (at most 10 pmol/m2-s).
As the aeration, a general aeration method can be used. The aeration 20 is not limited to a method for simply aerating a liquid with a gas, and includes
a method for introducing a gas by stirring or shaking.
Examples of the gas that can be used for aeration include air, pure oxygen, and a mixed gas of these gases.
[0061]
25 The temperature of the อาหารเพาะเลี้ยง in the culture step is not
specifically limited, as long as it is a temperature at which the ไมโครแอลจี can be grown. A culture temperature (temperature of the อาหารเพาะเลี