The culture supernatant was removed from the tube containing
nutrient broth by repeated centrifugation. Exactly 500 μl of bacterial
suspension ca. 1108 cfu/ml was taken in a multi-dish
containing a small block of P. capsici mycelia and then they were
kept in dark for 3 days. After three days, the multi-dishes were
kept for 20 min at 0 °C for cold-shock and then allowed to stand
for 1 h at room temperature for zoospore release. A control
treatment using sterilized water instead of culture supernatant of
bacteria was used. The number of zoospore released per ml of
water was counted (Islam et al., 2011). Each treatment was repeated
for three times.