The enumeration of particular microbial groups was performed
by using the following media (Scharlab, Barcelona, Spain) and
culture conditions: a) Nutrient Agar for total aerobic plate count,
pour-plated and incubated at 30 C for 48 h, and also for total
aerobic psychrotrophic count, pour-plated and incubated at 10 C
for 10 days; b) MEA for yeasts and moulds, pour-plated and incubated
at 25 C for 5 days; c) VRBD agar for total enterobacteria,
pour-plated, overlaid with the same medium and incubated aerobically
at 37 C for 48 h; d) MRS agar for lactic acid bacteria, pourplated
and incubated at 25 C for 5 days. The counts were performed
in triplicate.