glucose and
ethanol at varying yeast dry cell weight conditions of 10 (Fig. 5A),15
(Fig. 5B) and 26 mg (Fig. 5C) using 5 mg T. reesei culture supernatant.
Both cellobiose and glucose were completely consumed with
the addition of 10 mg yeast (corresponding to 2 CBU) in 12 h of
fermentation (Fig. 5A). Mannose consumption followed the same
pattern (data not shown). There was no significant difference in
sugar consumption observed with the addition of more yeast
(Fig. 5B and 5C). The sugar consumption pattern was similar
regardless of whether the supplementation of cellobiase activity
was achieved through the use of recombinant AaBGL1 or supplementation
with Novozyme 188 (Fig. 5AeC). Ethanol concentration
increased with time, achieving a peak at 72 h of fermentation in all
dry cell weight conditions, with no perceptible difference between
the different cell weight conditions (Fig. 5AeC). Similar to sugar
consumption, ethanol production efficiency did not differ between
recombinant AaBGL1 and Novozyme 188 (Fig. 5, and Table S3).