To evaluate the potential detachment of the embedded
silver nanoparticles from the silver–chitosan films, pieces
(3 cm 3 cm) of each corresponding film were placed in
100 ml of distilled water and analyzed over time with or
without sonication (Eumax, 60 Hz, up to 75 min) to quantify
the amount of silver released. The detection was carried out
by using UV–vis spectrophotometry.