Inject test solution (10-50 µL) into column with flow rate of mobile phase at 1.5-2.5 mL/min. Inject same volume of standard solution that will give peak response ±10% of analyte peak response. Two injections each of test and standard solution are required for adequate precision.
Measure areas or peak heights of each sugar peak in test solution and standard, but only measure peak height for components which are near detection limit and have adjacent interfering peak.