The shrimps from each treatment group in the primary quarantine were allocated to the corresponding dietary treatment in the 5-m3 tanks (2.5-m diameter) with three replicates for each diet. Each tank was stocked with five females and three to four males. After 2 months in secondary quarantine, eyestalk-ablation was applied to the molted females on the fourth day after molting and artificial insemination was applied to those females that were not naturally