2.2.1. Acid digestion for bone and organs
This procedure was studied for all sample tissues while
developing the method but for the biomonitoring study this
digestion was only used for the bone samples. About 0.25 g of
each dried tissue were mixed with 8.0 mL of 7.00 mol L1 HNO3
acid in PTFE vessels and left in predigestion for 30 min. Then, the
vessels were closed and the samples were digested employing the
microwave oven operating under the conditions shown in